In most cells, microtubules extend outward from the microtubule-organizing centers (MTOCs). In animal cells, the major microtubule-organizing center is the centrosome, located adjacent to the nucleus. Microtubules are also located in cilia and flagella of prokaryotes and human sperm cells.
Structure of Microtubules
Assembly and Disassembly of Microtubules
Functions of Microtubules
Composition
Each microtubule subunit comprises two closely related polypeptides: α-tubulin, and β-tubulin, forming heterodimers. Multiple units of these dimers polymerize to form a chain called the protofilament. Then, 13 protofilaments arrange into a cylindrical pattern to form a microtubule. In a microtubule, the subunits are organized such that they all face the same direction to form 13 parallel protofilaments. Thus, the microtubule is polar with the alpha-tubulin exposed at one end and beta-tubulin at the other.
Microtubules and Dynamic Instability
When new GTP-bound tubulin molecules add up more rapidly than GTP is hydrolyzed, the microtubule holds on to a GTP cap at its plus end, causing the microtubule to grow. In contrast, if the rate of polymerization decreases, then the GTP bound to tubulin at the plus end is hydrolyzed to GDP. Finally, the GDP-bound tubulin dissociates, resulting in rapid depolymerization and shrinkage of the microtubule. This phenomenon where microtubules alternate between cycles of growth and shrinkage is called dynamic instability. Maintaining Cell Shape Microtubules help maintain cell shape and stability with microfilaments and intermediate filaments. Together with the other cytoskeleton element, microtubules form an architectural framework that establishes the overall polarity of the cell. Cell Movement Cells provide structure to cilia and flagella and thus help move bacteria and other prokaryotes. Motile cilia and eukaryotic flagella have the canonical ‘9+2’ arrangement of microtubules where nine doublet microtubules surround a central pair of singlet microtubules. Microtubules in the trachea cells prevent mucus and dirt from entering the lungs. The fallopian tubes (female reproductive system) move the egg released from the ovary to the uterus. Cell Division During mitosis, microtubules play a crucial role in forming the mitotic spindle (spindle apparatus). The mitotic spindle helps to separate chromosomes during cell division so that the chromosomes can be partitioned equally into two daughter cells. The spindle apparatus also helps to form the contractile ring that separates the two daughter cells during cytokinesis. Three types of microtubules participate in mitosis: astral, polar, and kinetochore microtubules. Astral microtubules radiate from the MTOCs of a cell to the cell membrane, thus keeping the mitotic spindle in place. Polar microtubules link between two MTOCs and help separate chromosomes. Kinetochore microtubules attach to chromosomes that help to pull them apart. Intracellular Transport and Communication As part of the cytoskeletal network, microtubules help move organelles inside a cell’s cytoplasm. Microtubules also help the various cell components to communicate with each other. Forming an Internal Transport Network They form an internal transport network for moving materials throughout the cell and between the exterior and interior of the cell. This trafficking is done by microtubule-associated proteins kinesin and dynein.
